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Cell Imaging Facility
Description:
The Cell Imaging Facility offers state-of-the art instrumentation and services for the study of biological processes at the tissue, cellular and subcellular levels. The facility's services include light, fluorescence, confocal, and electron microscopy, microinjection, digitally controlled temperature stage for live cell observation, computerized image analysis, and digital image manipulation.

Equipment:

Light Microscopy
  • Zeiss LSM 510 META confocal microscope
  • Zeiss LSM 510 META confocal microscope with 405nm laser
  • Spinning Disc Confocal Microscope
  • Zeiss Axioskop Upright Microscope with CRi spectral CCD camera
  • Eppendorf Microinjection System
  • Total Internal Reflection Fluorescent (TIRF) Microscope
  • Nikon LiveScan Swept Field Confocal (SFC) Microscope
  • Nikon C-Si Spectral Imaging Laser Confocal System
Electron Microscopy
  • BAL-TEC MCS 010 Rotary Shadowing System
  • Dissecting and light microscopes
  • 35mm camera and copy stand
  • Protein spray apparatus
  • Tousimis Samdri 790 Critical Point Dyer
  • IEC Minotome Microtome-Cryostat
  • Six foot fume hood
  • Ovens - Ultramicrotomy - facilities are located in a 95 square foot room equipped with
    • Leica Ultracut UCT Ultramicrotome
    • Leica Ultracut E microtome
    • Reichert FC 4D Low Temperature Sectioning System
    • Two LKB 780 Knife makers
    • Leedal Wet Bench
  • JEOL 100CX-II Transmission Electron Microscope
  • JEOL JEM-1220 Transmission Electron Microscope
Services:
  • Training and technical support for the light, confocal, and electron microscopy
  • Preparation of samples for light, electron or confocal microscopy
Contact:

Director:
Teng-Leong Chew, PhD
t-chew@northwestern.edu

Location(s):
The Cell Imaging Facility is located on the second floors of the adjoining Searle and Morton buildings. It consists of a 16-room suite that occupies approximately 3,950 sf. This suite is tailor-designed to our detailed specifications, and every room is equipped with dimmer light, proper temperature control, directed air in- and outlet (to minimize air flow), and accessible by special keycards available only to authorized users. Within the facility, there are two "wet" laboratory rooms with fume hoods, one for EM sample preparation, one for our federally funded research work. The latter lab is also available for users who need immediate laboratory set-up to complete their time-dependent imaging experiments.

Phone:
312.503.4445

Website:
http://www.feinberg.northwestern.edu/cif/
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Clinical Cancer Center: 866.LURIE.CC || Chicago Administrative Office: 312.908.5250.
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