The Keck Biophysics Facility is a unique resource that provides researchers with 24-hour access to state of the art instruments. The facility is designed to facilitate biophysical and biochemical characterization of macromolecules. Services include use of fluorometers, spectrometers, calorimeters, imagers, batch and on- line light scattering instrumentation, an HPLC and a real-time PCR machine, fermentors.
Spectroscopy
- UV/Vis/NIR Spectrophotometer with Integrating Sphere (PE LAMBDA 1050)
- Circular Dichroism Spectrometer (J-815)
- Fluorescence Plate Reader (Gemini EM)
- Fluorescence Polarization (BEACON 2000)
- Frequency Domain Fluorescence Spectrometer (ISS K2)
- Photon -Counting Fluorescence Spectrometer (ISS PC1)
- Stopped-flow Spectrometer (APL SX18)
- UV/Vis DAD Spectrophotometer (HP 8452)
- Nanodrop 3300 Fluorimeter
Analytical
- Differential Scanning Calorimeter (VP-DSC)
- Isothermal Titration Calorimeter (ITC200)
- HPLC (Agilent 1100)
- Rapid Quench Flow (KinTek RQF3)
- Size Exclusion Chromatography with on-line Static and Dynamic Light Scattering (MALS/QELS) detectors
- Dynamic Light Scattering Instrument - batch mode (BI-9000/BI-200)
- Analytical Ultracentrifuge (XLA-70)
Imaging
- Gel Imaging System (Gel Logic 200)
- Phosphoimager/Fluoroimager (Typhoon 9400)
Other
- Isoelectric Focusing Gel Electrophoresis (Protean)
- Fermentor: 1.25L, 15L (BioFlo)
- Real Time PCR ( Bio-Rad ICycler)
- Rotor Type 45Ti
- Individual training
- Assistance with experimental design, sample preparation and data analysis
- Technical expertise, written manuals, guidelines and application notes
- Staff -service options available on most instruments
Location(s):
The Keck Facility is part of Northwestern University's Center for Structural Biology on the Evanston campus. Our office is located on the 3rd floor of Cook Hall and the instrument rooms are located on the 4th floor of Cook Hall. Cook Hall, 2220 Campus Drive, Evanston Campus
Phone:
847.491.7610
Website:
http://groups.molbiosci.northwestern.edu/keck/
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