The mission of the Mouse Histology & Phenotyping Laboratory (MHPL) is to assist investigators with gross and histological characterization of genetically modified murine models. The laboratory provides comprehensive histology services for all rodent species (mouse, rat, etc.). This includes frozen and paraffin sectioning as well as standard and special staining techniques. Histopathological analysis can be provided for single organs or they can involve a systemic overview of all major organ systems to identify histopathologic abnormalities that may exist. Pathologist consultation can also be provided to help develop strategies to elucidate phenotypes and gain mechanistic insight regarding the biologic actions of the targeted molecule or toxicity of exogenously administered substances. In addition, the laboratory provides training opportunities for learning histology techniques and phenotyping analysis.
Click here to view charge rates
- Leica ASP300S Tissue Processor
- Leica EG1160 Embedding Center
- Leica AutoStainer XL Automated Stainer
- Leica CV5000 Automated Glass Coverslipper
- Microm HM 355S Fully Automated Motorized Rotary Microtome
- Microm HM 325 Rotary Microtome
- Olympus BX41 Stereo Compound Microscope with Dual view side observation unit (two headed scope)
- Spot Insight Hi-resolution Digital Camera Model 18.2
- Leica MZ95 Dissecting Scope
Pathology
- Necropsy and tissue collection
- Gross exam with report
- Histopathology (slide interpretation)
- Toxicity evaluation in pathology
- Photo documentation of lesions
- Training in necropsy technique including perfusion of mice and special tissue dissection
- Phenotype screening (complete necropsy and histopathology of mutant mice)
- Assistance with development of animal pathology protocols
Histology
- Automated tissue processing and paraffin embedding
- Paraffin sectioning with rotary microtomes
- Serial and levels sectioning
- Standard hematoxylin & eosin staining
- Unstained slides for subsequent analysis
- Special stains e.g. Trichrome, PAS, Luxol fast blue, Cresyl violet, and many other stains
- Consultation
Director:
Warren Tourtellotte, MD, PhD
warren@northwestern.edu
Associate Director:
Lin Li, MD,MS
l-li@northwestern.edu
Facility Manager:
Donna Emge
d-emge@northwestern.edu
Location(s):
710 N. Fairbanks Court; Olson 8335.
Phone:
312.503.2679
Website:
http://www.feinberg.northwestern.edu/research/cores/units/mouse-histology.html
|
|
