The mission of the Northwestern University Mouse Histology & Phenotyping Laboratory (MHPL) is to assist investigators with mouse histopathology. The laboratory provides comprehensive histology services for all rodent species (i.e., mouse, rat, etc.) which include necropsy, phenotyping of organs and tissues, dissection and tissue processing, and one-on-one consultation. The lab generates unstained paraffin and frozen sections for investigators to be used for special staining and immunohistochemistry (IHC). Pathologist consultation can also be provided to help develop strategies to elucidate phenotypes and gain mechanistic insight regarding the biologic actions of the targeted molecule or the toxicity of exogenously administered substances. In addition, the laboratory provides training opportunities for learning histology techniques and phenotyping analysis.

Click here to view Charge Rates
Click here to view Histology & Immunochemistry Requisition
Click here to view Mouse Necropsy Requisition
Click here to view Slide Interpretation Requisition
Click here to view Primary Antibody List (4/2/2013)

Equipment:

• Leica ASP300S Tissue Processor
• Leica EG1160 Embedding Center
• Leica AutoStainer XL Automated Stainer
• Leica CV5000 Automated Glass Coverslipper
• Microm HM 355S Fully Automated Motorized Rotary Microtome
• Microm HM 325 Rotary Microtome
• Olympus BX41 Stereo Compound Microscope with Dual view side observation unit (two headed scope)
• Spot Insight Hi-resolution Digital Camera Model 18.2
• Leica MZ95 Dissecting Scope
• Nikon SMZ-1500 Zoom Stereo Microscope System with Epi-Fluorescence Attachment
• Biocare IntilliPATH automated Immunohistochemistry (IHC) System

• Necropsy and tissue collection
• Gross exam with report
• Histopathology (slide interpretation)
• Histopathology toxicity evaluation
• Photo documentation of histopathology
• Training in necropsy techniques including perfusion of mice and special tissue dissection
• Phenotype screening (complete necropsy and histopathology of mutant mice)
• Assistance with development of animal pathology protocols
• Immunohistochemistry (IHC) - Using tyramide signal amplification (TSA) and ABC methods (automated), with DAB chromogenic substrate. See documents below for details.
• Freezing and embedding of tissues for histology
• Frozen sections
• Paraffin block processing and Paraffin sections
• Hematoxylin and Eosin staining of sections
• Specialized histochemical staining of sections (e.g. Trichrome, PAS, Luxol fast blue, Cresyl violet, and many other stains)
• Consultation

Contact:

General Contact
(312) 503-2679 (Histology)
(312) 503-2695 (Phenotype & IHC)
(312) 503-2369 (FAX)

Lin Li, MD, MS
Associate Director
l-li@northwestern.edu

Donna Emge ASCP-HT
Manager
d-emge@northwestern.edu

Warren Tourtellotte, MD, PhD, FACP
Director
warren@northwestern.edu

Location(s):
710 N. Fairbanks Court; Olson 8335.

Website:
http://www.feinberg.northwestern.edu/research/cores/units/mouse-histology.html